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Image Search Results
Journal: Scientific Reports
Article Title: Spatially-resolved intracellular sensing of hydrogen peroxide in living cells
doi: 10.1038/srep16929
Figure Lengend Snippet: ( a–d ) H 2 O 2 (200 μM) was added below the cells in the field of view using a microloader pipette tip. Images were recorded at a rate of 1 frame every 0.55 seconds with a spinning disk confocal microscope. The pseudocolor images (0–256 scaling, red is the greatest change) represent the ratio of emission at 516 nm obtained from excitation at 488 nm versus 405 nm. The scale bar is 20 μm. ( e ) Kinetics of oxidation obtained from regions of interest (white circles) within three cells.
Article Snippet: For HeLa cells, H 2 O 2 was added to the existing medium in the optical dish using either regular pipette tips (LTS-20, Mettler-Toledo, Greifensee, Switzerland) before imaging ( ) or 20 μL
Techniques: Transferring, Microscopy